How can plants and animals with desired characteristics be developed by selective breeding. Micropropagation of orchids, 3 volume set, 3rd edition. Micropropagation offers the potential to produce thousands or even millions of plants per annum. Inthinking promotes and supports the development of high quality educational resources which incorporate cuttingedge ideas, encourage independent thinking, and help students to prepare effectively for exams. When a plant cutting is used to reproduce asexually in the native environment it is called vegetative propagation. A1 micropropagation of plants using tissue from the shoot. Biologyhigher level first assessments 2016 last assessments 2022. Micropropagation is a reliable technology applied commercially worldwide for. Ibworldme is a resource site for the 2016 ib biology curriculum.
Explants may be obtained from normally pot grown plants. The new shoots are then separated and cultured, and the cycle is repeated until finally the shoots are transferred to a medium that promotes root development, to produce plantlets. Micropropagation biology, biology lessons, biology revision. Determinate growth indeterminate growth growth that stops. Auxin influences cell growth rates by changing the pattern of gene expression.
Micropropagation is a technique used to produce large numbers of identical plants clones from a selected stock plant. U4 plant shoots respond to the environment by tropisms. How can you estimate the uncertainty of any apparatus. Pdf in vitro studies on cassava plant micropropagation of. The propagation of selected plant through micropropagation would be useful for raising plantation using apical and nodal segment.
I would like to personally say that you have taught me biology and i want to. Following renovation in late 2015 the facilities of the molecular biology laboratory have been expanded and improved to keep pace with the increasing importance of molecular data in botany, conservation and horticulture. Photoautotrophic micropropagation using sugarfree medium is an area of potential utility to scaleup potato micropropagation for commercialization, and recent advancements in the development and utilization of large culture vessels and case studies concerning photoautotrophic micropropagation were described by zobayed et al. The micropropagation of plants solved many phytosanitary problems and allowed. Brand new ib teaching support matched to the new syllabus. Ib biology course book 2014 edition oxford ib diploma. Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of diseasefree plants and its merits and demerits.
Plants adapt their growth to environmental conditions how do the cells of the plant respond to hormonal and environmental stimuli. Multiplication of genetically identical copies of a cultivar by asexual reproduction is called clonal propagation. This video is for edexcel igcse biology 91 but is relevant for many gcse biology courses. Process of micropropagation in plant tissue culture. Plants can be propagated by sexual through generation of seeds or asexual through multiplication of vegetative parts means. Plants can reproduce asexually from meristems because they are undifferentiated cells capable of indeterminate growth. Monocotyldonea, dicotyldonea stem and root xylem diagrams. Key stage 5 sessions these programmes are planned in detail and. The journal provides original research devoted to the advancement and dissemination of basic and applied.
Micropropagation of plants using tissue from the shoot apex. It is a large scale production of the micro propagules within a small space. For various micropropagation procedures, any plant parts can be utilized in light of the fact that each cell can recover. Unit ii biotechnology and plants lesson 7 plant tissue culture 169 cells and tissue the starting point for micropropagation can be individual cells or small amounts of plant tissue. Correct use of terminology is a key skill in biology. Hi im looking for a oxfords ib biology course companion by allott and mindorff, for the new syllabus in ebookpdf version.
A textbook of physiology for medical students and physicians. Micropropagation of plants using tissues from the shoot apex, nutrient. Unit ii biotechnology and plants plant tissue culture 7. It contains a great amount of information including the most up to date scientific results.
Micropropagation definition of micropropagation by the. U2mitosis and cell division in the shoot apex provide cells needed for extension of the stem and development. The explants are firstly to be washed comprehensively with tap water and then with routinely used normal house hold detergent to remove all the traces of dust particles. The book offers a useful resource for horticulturists, researchers, commercial companies, plant propagators, biotechnologists. Sample questions international baccalaureate diploma programme subject brief sciences. Tutorial, auxin animation, extra auxin uk ppt green book updating. Ib biology refers to the international baccalaureate ib group 4 experimental science biology. Free access to more than biology links, downloadable free content and a variety of resources to aid both teacher and students. U5 auxin efflux pumps can set up concentration gradients of auxin in plant tissue. The propagation environment, biology of plants, development of seeds, seed selection, seed production and handling, principles of propagation from seeds, techniques of propagation by seeds and cuttings, principles of grafting and budding, techniques of grafting, propagation by specialized stems and roots, principles and practices of clonal selection, principles of tissue culture and micropropagation, techniques for micropropagation and grafting. The prodigious advantages of in vitro aseptic technique of clonal propagation i. We want you to know you have taught us so much and we want to thank you for every slide you made. For micropropagation of carnation, explants of about 1.
Diagram quiz on steps in recombinant dna or rdna technology biology multiple choice quizzes. Ib biology course book 2014 edition oxford ib diploma program ebookpdfversion. This is particularly valuable in the case of difficulttoroot species and those species like the palms which can neither be rooted by cuttings nor grafted because they lack a cambium. Adaxialthe side or on the side toward an axis such as on the upper surface of a. Micropropagation, genetic engineering, and molecular. A good model of a porous pot potometer link to supplier. Your ib biology course book to help you gauge your progress and understanding, the answers for the databased questions in your ib biology course book are available here. Since the first book on plant tissue culture by prof. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods. Micropropagationomicsgroupjournal of biotechnology and.
Ib mission statement the international baccalaureate aims to develop inquiring, knowledgeable and caring young people who help to create a better and more peaceful world through intercultural understanding and respect. Plant cells for micropropagation may be newly transformed as a result of genetic engineering, or they might be cells taken from plant tissue. Micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. Cloning plants micropropagation tissue culture gcse. Plantbased medicines play an important role in all cultures, and have been indispensable in maintaining health and combating diseases. Micropropagation of plants using tissue from the shoot apex, nutrient agar gels and growth. Ib biologyintroduction wikibooks, open books for an. All definitions and concept from chapter 9 plant science youd need to know for the ib exams. It covers these specific objectives from the syllabus 5. Carman abaxialthe side or on the side away from an axis such as on the lower surface of a leaf.
To this end the organization works with schools, governments and international organizations to develop. In vitro studies on cassava plant micropropagation of cassava manihot esculenta crantz. Ib biology core additional higher level ahl options bisv revision giving back biokqqanswers this work is licensed under a creative commons attributionnoncommercialsharealike 4. Please nte that this is now replaced by the 2009 examinations and the content of the pages are out of date. Typically, this involves culturing excised meristematic tissue on a special medium that encourages axillary bud development. Review notes for topic 2 molecular biology for higher and standard level ib international baccalaureate biology. When you book a school visit at wakehurst, let us know which exam board you are using and we will work with you to tailor the session for your students.
Once a predetermined structure has fully formed after a certain size is reached once the adult structure has. Biology lessons ap biology molecular biology teaching biology genome project dna project project ideas projects. Set up explants for african violet micropropagation lab hw. Biozone produces high quality resources for high school biology in the us grades 912. The international baccalaureate organization known as the ib offers four highquality. Culture media and vessels techniques and procedures for both orchids which were previously cultured and for those which were not plant hormones and growth regulators media. The following is or will hopefully eventually be an explanation of the entire ib biology syllabus. Micropropagation depends on the totipotency of plant tissue. What would the units be of the rate of transpirationcarry out the activities on the simple transpiration experiments worksheet. Micropropagation is a rapid multiplication of a selected plant using in vitro culture techniques.
You have made it possible for us to learn the content of biology and pass our many tests and exams. This is an animated powerpoint presentation that covers section of 5. The technique of plant tissue culture is used for growing isolated plant cells, tissues and organs under axenic conditions in vitro to regenerate and propagate entire plants. Undifferentiated cells in the meristems of plants allow intermediate growth. Plant tissue culture offers new ways for the improvement of this crop after many years of recalcitrance. U6 auxin influences cell growth rates by changing the pattern of gene expression. A stock plant is identified that often has some desirable feature. International baccalaureate diploma programme subject brief. Igcse biology selective breeding and cloning presentation. White in 1943, several volumes describing different aspects of ptc have been published. Diverse issues additionally emerge amid micropropagation of roses and their control is essential generally entire exertion will be futile. Micro propagation is techniques require the setting up of the laboratory and it and it has to be maintained well.
Tissue culture serves as an alternative to vegetative propagation by conventional methods, such as by rooting of cuttings. The in vivo clonal propagation is often difficult, expensive and even unsuccessful. Micropropagation in the twentyfirst century springer nature. Environmental plant biology at lancaster university. In this chapter various aspects of micropropagation have been discussed. Divided into three volumes, micropropagation of orchids third edition retains the exhaustive list of micropropagation protocols for many genera and updates each section to include new andor revised information about. Protocols for micropropagation of selected economicallyimportant. A tissue culture technique for plant propagation in which tissue is taken from a plant and grown in a laboratory to produce plantlets that are. Remember chikis biology is created to spread the knowledge of life sciences and biology by sharing all this free biology lectures video and a4 size pages presented by ankur kumar bhogle on you tube. Micropropagation of orchids, 2nd edition, is a valuable book of reference for people working in the area of orchid cultivation and for all who are enthusiastic with orchids. On behalf of my ib bio hl class we are all grateful for your site. The identification of active principles and their molecular targets from traditional medicine provides an enormous opportunity for drug development. Micropropagation of plants using tissue from the shoot apex, nutrient agar gels and growth hormones. This chapter summarizes the application of the most commonly used in vitro propagation techniques for trees, shrubs and herbaceous species.